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human recombinant full length usp7  (R&D Systems)


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    Structured Review

    R&D Systems human recombinant full length usp7
    Human Recombinant Full Length Usp7, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+usp7/Recombinant+Human+His8-USP7+Protein%2C+CF/pm41786746-232-0-7
    Average 94 stars, based on 15 article reviews
    human recombinant full length usp7 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: USP7 deubiquitinase stabilizes FAN1 to support DNA crosslink repair and suppress CAG repeat expansion.
    Article Snippet: After sonication and centrifugation, GST-tagged FAN1 was purified from soluble extracts using Glutathione Sepharose AR TI CL E IN P RE SS 4 Fast Flow beads (GE Healthcare). .. GST fusion proteins bound to glutathione beads were mixed with 0.5 μg of human recombinant USP7 (R&D Systems) and incubated for 1 h at 4 °C in 1 ml of PBS-1% Triton. ..

    Article Title: Deubiquitination of NF-?B by Ubiquitin-Specific Protease-7 promotes transcription
    Article Snippet: .. Ubiquitinated GST-p65 was added to a deubiquitination reaction (50 mm Tris⋅HCl, pH 7.4, 150 mm NaCl, 5 mM MgCl 2 , and 10 mm DTT) with or without purified insect-derived recombinant USP7 (E-519–025 RnD Systems). ..

    Article Title: USP7 deubiquitinase stabilizes FAN1 to support DNA crosslink repair and suppress CAG repeat expansion
    Article Snippet: After sonication and centrifugation, GST-tagged FAN1 was purified from soluble extracts using Glutathione Sepharose 4 Fast Flow beads (GE Healthcare). .. GST fusion proteins bound to glutathione beads were mixed with 0.5 μg of human recombinant USP7 (R&D Systems) and incubated for 1 h at 4 °C in 1 ml of PBS-1% Triton. ..

    Article Title: USP7 deubiquitinase stabilizes FAN1 to support DNA crosslink repair and suppress CAG repeat expansion
    Article Snippet: For GST pull-down assays, GST fusion plasmids were transformed in BL21 RIL (CodonPlus) E. coli (Stratagene) and recombinant proteins were expressed by incubating the bacteria for 24 h at 16 °C after the addition of 100 μM IPTG. .. Beads were then mixed with 0.5 μg of human recombinant USP7 (R&D Systems) and incubated rotating for 2 h at 4 °C. .. After sonication and centrifugation, GST-tagged FAN1 was purified from soluble extracts using Glutathione Sepharose 4 Fast Flow beads (GE Healthcare).

    Incubation:

    Article Title: USP7 deubiquitinase stabilizes FAN1 to support DNA crosslink repair and suppress CAG repeat expansion.
    Article Snippet: After sonication and centrifugation, GST-tagged FAN1 was purified from soluble extracts using Glutathione Sepharose AR TI CL E IN P RE SS 4 Fast Flow beads (GE Healthcare). .. GST fusion proteins bound to glutathione beads were mixed with 0.5 μg of human recombinant USP7 (R&D Systems) and incubated for 1 h at 4 °C in 1 ml of PBS-1% Triton. ..

    Article Title: USP7 deubiquitinase stabilizes FAN1 to support DNA crosslink repair and suppress CAG repeat expansion
    Article Snippet: After sonication and centrifugation, GST-tagged FAN1 was purified from soluble extracts using Glutathione Sepharose 4 Fast Flow beads (GE Healthcare). .. GST fusion proteins bound to glutathione beads were mixed with 0.5 μg of human recombinant USP7 (R&D Systems) and incubated for 1 h at 4 °C in 1 ml of PBS-1% Triton. ..

    Article Title: USP7 deubiquitinase stabilizes FAN1 to support DNA crosslink repair and suppress CAG repeat expansion
    Article Snippet: For GST pull-down assays, GST fusion plasmids were transformed in BL21 RIL (CodonPlus) E. coli (Stratagene) and recombinant proteins were expressed by incubating the bacteria for 24 h at 16 °C after the addition of 100 μM IPTG. .. Beads were then mixed with 0.5 μg of human recombinant USP7 (R&D Systems) and incubated rotating for 2 h at 4 °C. .. After sonication and centrifugation, GST-tagged FAN1 was purified from soluble extracts using Glutathione Sepharose 4 Fast Flow beads (GE Healthcare).

    Purification:

    Article Title: Deubiquitination of NF-?B by Ubiquitin-Specific Protease-7 promotes transcription
    Article Snippet: .. Ubiquitinated GST-p65 was added to a deubiquitination reaction (50 mm Tris⋅HCl, pH 7.4, 150 mm NaCl, 5 mM MgCl 2 , and 10 mm DTT) with or without purified insect-derived recombinant USP7 (E-519–025 RnD Systems). ..

    other:

    Article Title: USP7 deubiquitinase stabilizes FAN1 to support DNA crosslink repair and suppress CAG repeat expansion.
    Article Snippet: After sonication and centrifugation, GST-tagged FAN1 was purified from soluble extracts using Glutathione Sepharose AR TI CL E IN P RE SS 4 Fast Flow beads (GE Healthcare).



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